Role of Sarcolemmal BK_<Ca> Channels in Stretch-Induced Extrasystoles in Isolated Chick Hearts

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概要

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• Background: It remains unclear whether sarcolemmal BKCa channels in post-hatch chick ventricular myocytes contribute to stretch-induced extrasystoles (SIE), and whether they are stretch-activated BKCa (SAKCa) channels or a non-stretch-sensitive BKCa variant. Methods and Results: To determine the role of sarcolemmal BKCa channels in SIE and their stretch sensitivity, an isolated 2-week-old Langendorff-perfused chick heart and mathematical simulation were used. The ventricular wall was rapidly stretched by application of a volume change pulse. As the speed of the stretch increased, the probability of SIE also significantly increased, significantly shortening the delay between SIE and the initiation of the stretch. Application of 100nmol/L of Grammostola spatulata mechanotoxin 4, a cation-selective stretch-activated channel (SAC) blocker, significantly decreased the probability of SIE. The application of Iberiotoxin, however, a BKCa channel blocker, significantly increased the probability of SIE, suggesting that a K+ efflux via a sarcolemmal BKCa channel reduces SIE by balancing out stretch-induced cation influx via SACs. The simulation using a cardiomyocyte model combined with a new stretch sensitivity model that considers viscoelastic intracellular force transmission showed that stretch sensitivity in BKCa channels is required to reproduce the present wet experimental results. Conclusions: Sarcolemmal BKCa channels in post-hatch chick ventricular myocytes are SAKCa channels, and they have a suppressive effect on SIE. (Circ J 2011; 75: 2552-2558)

• 社団法人　日本循環器学会の論文
• 2011-10-25

著者

• Iribe Gentaro

  Cardiovascular Physiology Okayama University Graduate School Of Medicine Dentistry And Pharmaceutica

• JIN Honghua

  Cardiovascular Physiology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceuti

• NARUSE Keiji

  Cardiovascular Physiology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceuti

• Naruse Keiji

  Cardiovascular Physiology Okayama University Graduate School Of Medicine Dentistry And Pharmaceutical Sciences

• Jin Honghua

  Cardiovascular Physiology Okayama University Graduate School Of Medicine Dentistry And Pharmaceutical Sciences

• Naruse Keiji

  Cardiovascular Physiology Graduate School Of Medicine Dentistry And Pharmaceutical Sciences Okayama University

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