Spatio-Temporal Control of Bacterial-Suspension Luminescence Using a PDMS Cell

概要

論文の詳細を見る
The aim of this study was to realize the spatially uniform illumination of a bacterial suspension using polydimethylsiloxane (PDMS)–made cells and to demonstrate the potential of a “quorum sensing reactor array.” After optimizing the condition to realize uniform luminescence, bioluminescent bacterial suspensions with different cell densities were encapsulated in 12 PDMS capsules and placed clockwise. Even without stirring, 11 capsules were proved to start/finish illumination in the order of cell density (ratio ranging 20–2−10). To realize such an array, spatially uniform bioluminescence was achieved with a geometrically symmetric supply of oxygen. A PDMS cell was suitable for this purpose. Suspensions with a depth of less than 2.71 mm showed spatially uniform luminescence from the beginning to the end without convection. In this study, for the first time, we provide an insight into an inhomogeneous suspension of bioluminescent bacteria. Understanding bacterial behavior at a suspension level in addition to the genetic and cellular levels would provide complementary information to illustrate the entire picture of bacterial function.
社団法人化学工学会の論文
2010-12-01