Analysis of plasma protein in newborn rats treated with diethylstilbestrol by using rapid micro two-dimensional electrophoresis
スポンサーリンク
概要
- 論文の詳細を見る
We examined the effect of diethylstilbestrol(DES) administration on newborn male rats by rapid micro two-dimensional polyacrylamide gel electrophoresis (RM2D-PAGE) under non-denaturing/denaturing conditions and a 10-17% second-dimensional gel. We began by visually analyzing the changes at each week over time (weeks 1-9) in plasma protein spots from the same individual DES-administered male rats. We found the tendency for mercaptoalbumin (reduced form) spots to increase in week 1 in DES-administered rats. We also analyzed the quantitative alterations in α2u-globulin (AUG), a biomarker of endocrine disruptors, and found a two-week delay in expression in DES-administered rats in comparison to those not given DES (control rats). We then investigated the reproducibility of these results by using samples taken from multiple male rats in weeks 5, 7 and 9 to perform electrophoretic analysis three times for each individual, and observed the changes in AUG. AUG production was not seen in week 5 in the control rats, but was present at weeks 7 and 9. In DES-administered rats, expression was not observed at week 5 or 7, but was present at week 9. In addition, we identified the spots of AUG by Western blotting after electrophoresis. We consequently confirmed that AUG production delayed by two weeks in DES-administered rats, in comparison with control rats, which suggests that the effect of either a decline in AUG synthesis function or its depletion results in stunting of the reproductive organs, which was confirmed through autopsy.
- 2009-12-01
著者
-
SAKAGUCHI Kazuko
Department of Health Care Chemistry, College of Environmental Health, Azabu University
-
SUZUKI Jun
Department of Health Care Chemistry, College of Environmental Health, Azabu University
-
Suzuki Jun
Department Of Cardiovascular Medicine Tohoku University Graduate School Of Medicine
-
Sakaguchi Kazuko
Department Of Food Biochemistry School Of Life And Environmental Science Azabu University
-
Honda Masayuki
Department Of General Surgery Fukuoka Dental College Hospital
-
Honda Masayuki
Department Of Hygiene School Of Life And Environmental Science Azabu University
-
UJIIE Shogo
Department of Food Biochemistry, School of Life and Environmental Science, Azabu University
-
KIRA Ryo
Department of Food Biochemistry, School of Life and Environmental Science, Azabu University
-
KAKUNO Yoichi
Department of Hygiene, School of Life and Environmental Science, Azabu University
-
Kira Ryo
Department Of Food Biochemistry School Of Life And Environmental Science Azabu University
-
Ujiie Shogo
Department Of Food Biochemistry School Of Life And Environmental Science Azabu University
-
Kakuno Yoichi
Department Of Hygiene School Of Life And Environmental Science Azabu University
-
Honda Masayuki
Department Of Applied Chemistry Faculty Of Engineering Okayama University Of Science
-
Suzuki Jun
Department Of Cardiology Saiseikai Saijo Hospital
-
Suzuki Jun
Department Of Food Biochemistry School Of Life And Environmental Science Azabu University
関連論文
- Long-Term Epoprostenol Therapy in Pulmonary Artery Hypertension : Sequence of Changes in Hemodynamic Effects(Pulmonary Circulation)
- OE-160 Evaluation of Drug Eluting Stent Fracture in RCA Lesion by 64-slice Multislice Computed Tomography(OE27,CT/MRI (Coronary/Vascular) 1 (I),Oral Presentation (English),The 73rd Annual Scientific Meeting of The Japanese Circulation Society)
- Micro two-dimensional electrophoretic analysis of changes in plasma proteins of next-generation rat of the pregnant rats administered to coplanar PCBs
- Association of monocyte chemoattractant protein 1 gene polymorphism with susceptibility to nonfamilial idiopathic dilated cardiomyopathy
- FRS-024 Impact of Synergistic Polymorphisms in Adrenergic Receptor Related Genes and Cardiovascular Events in Patients with Dilated Cardiomyopathy(FRS5,Novel Biomarkers in Heart Failure (M),Featured Research Session (English),The 73rd Annual Scientific Me
- OJ-119 Synergistic Polymorphisms of Angiotensin Converting Enzyme and Endothelial Nitric Oxide Synthase and Cardiovascular Events in Patients with Hypertrophic Cardiomyopathy(Cardiomyopathy, basic / clinical(02)(M),Oral Presentation (Japanese),The 72nd An
- OE-037 Combined Analysis of Polymorphisms in Inflammation-Related Genes and Their Effects on Atrial fibrillation in Patients with Dilated Cardiomyopathy(Arrhythmia, diagnosis/Pathophysiology/EPS(01)(A),Oral Presentation(English),The 72nd Annual Scientific
- PJ-606 Pulmonary Veno-occlusive Disease : Acute Vasodilator Response and Prognosis(PJ102,Pulmonary Circulation 2 (H),Poster Session (Japanese),The 73rd Annual Scientific Meeting of The Japanese Circulation Society)
- PE-445 Renin-Angiotensin System Plays an Important Role in Diabetes Mellitus-Induced Diastolic Dysfunction in Hypertensive Rats(PE075,Heart Failure (Pathophysiology) (M),Poster Session (English),The 73rd Annual Scientific Meeting of the Japanese Circulati
- FRS-024 Impact of Synergistic Polymorphisms in Adrenergic Receptor Related Genes and Cardiovascular Events in Patients with Dilated Cardiomyopathy(FRS5,Novel Biomarkers in Heart Failure (M),Featured Research Session (English),The 73rd Annual Scientific Me
- OJ-119 Synergistic Polymorphisms of Angiotensin Converting Enzyme and Endothelial Nitric Oxide Synthase and Cardiovascular Events in Patients with Hypertrophic Cardiomyopathy(Cardiomyopathy, basic / clinical(02)(M),Oral Presentation (Japanese),The 72nd An
- OE-037 Combined Analysis of Polymorphisms in Inflammation-Related Genes and Their Effects on Atrial fibrillation in Patients with Dilated Cardiomyopathy(Arrhythmia, diagnosis/Pathophysiology/EPS(01)(A),Oral Presentation(English),The 72nd Annual Scientific
- Intracoronary Administration of a Thromboxane A_2 Synthase Inhibitor Relieves Acetyicholine-Induced Coronary Spasm
- Circadian variation of vascular endotherial function - correalation with neurohormonal agents
- Magnesium Deficiency in Patients With Recent Myocardial Infarction and Provoked Coronary Artery Spasm
- Beneficial Effect of Troglitazone, an Insulin-Sensitizing Antidiabetic Agent, on Coronary Circulation in Patients With Non-Insulin-Dependent Diabetes Mellitus
- Subacute and Chronic Effects of DDD Pacing on Left Ventricular Diastolic Function in Ptients With Non-Obstructive Hypertrophic Cardiomyopathy
- Clinical Characteristics of Female Patients With Coronary Spastic Angina : Comparison With Male Patients
- Analysis by High-performance Anion-exchange Chromatography of Component Sugars as Their Fluorescent Pyridylamino Derivatives(Analytical Chemistry)
- Improved Method for Fluorescence Labeling of Sugar Chains with Sialic Acid Residues(Analytical Chemistry)
- Diabetes mellitus accelerates left ventricular diastolic dysfunction through activation of the renin-angiotensin system in hypertensive rats
- Portopulmonary Hypertension : Hemodynamics, Pulmonary Angiography, and Configuration of the Heart
- PJ-083 Differentiation of Hypertrophic Cardiomyopathy from Hypertensive Heart Disease Using Two-Dimensional Strain Analysis(PJ014,Cardiomyopathy/Hypertrophy (Clinical) (M),Poster Session (Japanese),The 73rd Annual Scientific Meeting of The Japanese Circul
- PE-548 Clinical Implication of Global Strain Rate during Isovolumic Relaxation Time in Patients with Non-ischemic Dilated Cardiomyopathy(PE092,Echo/Doppler (Myocardium) (I),Poster Session (English),The 73rd Annual Scientific Meeting of the Japanese Circul
- PE-114 A Novel Method for Measurement of Carotid Arterial Elastic Properties Using Two Dimensional Speckle Tracking Analysis(PE019,Echo/Doppler (New Technology) (I),Poster Session (English),The 73rd Annual Scientific Meeting of The Japanese Circulation So
- OE-130 A Link between Catenoid Shape Configuration of Hypertrophied Septum and Regional Myocardial Function in Patients with Hypertrophic Cardiomyopathy(OE22,Echo/Doppler (Myocardium) (I),Oral Presentation (English),The 73rd Annual Scientific Meeting of T
- OE-127 Right Ventricular Septal Pacing Preserves Left Ventricular Synchrony and Torsional Behavior in Comparison with Conventional Apical Pacing(OE22,Echo/Doppler (Myocardium) (I),Oral Presentation (English),The 73rd Annual Scientific Meeting of The Japan
- PJ-704 Possible Link Between Left Ventricular Systolic and Diastolic Dyssynchrony and Diastolic Function in Patients With Essential Hypertension(Hypertension, clinical(09)(H),Poster Session(Japanese),The 72nd Annual Scientific Meeting of the Japanese Circ
- INVOLVEMENT OF GLUCOCORTICOID IN INSULIN-INDUCED ANGIOGENESIS OF ADJUVANT POUCH GRANULOMA IN DIABETIC MICE
- QUANTITATIVE METHOD FOR MEASURING ADJUVANT-INDUCED GRANULOMA ANGIOGENESIS IN INSULIN-TREATED DIABETIC MICE
- MOUSE GRANULOMA POUCH INDUCED BY FREUND'S COMPLETE ADJUVANT WITH CROTON OIL
- OE-117 Eplerenone, a Selective Aldosterone Receptor Antagonist, Inhibits Atherosclerotic Lesion Formation in Apolipoprotein E-Deficient Mice(Atherosclerosis, Basic 1 (IHD) : OE14)(Oral Presentation (English))
- Role of interferon regulatory factor-1 (IRF-1) in the regulation of apoptosis in vascular remodeling; study using IRF-1 null mice
- Efficacy of Acute Inhalation of Nitric Oxide in Patients With Primary Pulmonary Hypertension Using Chronic Use of Continuous Epoprostenol Infusion
- Possible Effectiveness of Combination Therapy in Patients with Severe Pulmonary Hypertention
- PE-337 Inhibition of Rho-kinase Ameliorates Diastolic Heart Failure in Hypertensive Rats(Heart failure, basic(03)(M),Poster Session(English),The 72nd Annual Scientific Meeting of the Japanese Circulation Society)
- OE-135 Diabetes Mellitus Accelerates Left Ventricular Diastolic Dysfunction through Activation of Renin-angiotensin System in Hypertensive Rats(Cardiac function, basic/clinical(01)(M),Oral Presentation(English),The 72nd Annual Scientific Meeting of the Ja
- PE-436 Rho-kinase Pathway Plays an Important Role in the Pathogenesis of Diastolic Heart Failure in Dahl Salt-Sensitive Hypertensive Rats(Heart failure, basic-4, The 71st Annual Scientific Meeting of the Japanese Circulation Society)
- OE-205 Impact of Diabetes Mellitus on Left Ventricular Diastolic Dysfunction in Dahl Salt-Sensitive Hypertensive Rats(Cardiac function, basic/clinical-1, The 71st Annual Scientific Meeting of the Japanese Circulation Society)
- The Outcome of Long-term Epoprostenol Therapy in Patients with Pulmonary Arterial Hypertension (Pulmonary Circulation 4 (H), The 69th Annual Scientific Meeting of the Japanese Circulation Society)
- PE-169 Stent Underexpansion is Not Main Reason of In-Stent Restenosis after Silorimus-Eluting Stents Implantation with RCA Lesion(Coronary revascularization, PCI(09)(IHD),Poster Session(English),The 72nd Annual Scientific Meeting of the Japanese Circulati
- 5 Current Status and Future Direction of Multi Detector-row Computed Tomography for the Integrated Management of Coronary Artery Disease(Symposium 14 (SY-14) (I) New Horizon of CT, MR and PET in Diagnostic Cardiac Imaging,Special Program,The 72nd Annual S
- FRS-067 Microtubule Polymerization Plays an Important Role in the Pathogenesis of Cardiomyocyte Apoptosis(Heart Failure, Cardiac Remodeling and Cardiomyopathy 1, The 71st Annual Scientific Meeting of the Japanese Circulation Society)
- Metal(CoSi_2)/Insulator(CaF_2) Resonant Tunneling Diode
- Epoprostenol Infusion Therapy Changes Angiographic Findings of Pulmonary Arteries in Patients With Idiopathic Pulmonary Arterial Hypertension
- OE-072 Pathogenetic Link between Microtubule Polymerization and Myocyte Apoptisis : Implication for Heart Failure(Cell death, apoptosis/necrosis (M) OE12,Oral Presentation (English),The 70th Anniversary Annual Scientific Meeting of the Japanese Circulatio
- Colchicine, a Microtubule Depolymerizing Agent, Inhibits Myocardial Apoptosis in Rats
- PE-442 C-Reactive Protein Predicts Recurrence of Atrial Fibrillation in Patients with Hypertrophic Cardiomyopathy(PE074,Cardiomyopathy/Hypertrophy (Clinical) 2 (M),Poster Session (English),The 73rd Annual Scientific Meeting of the Japanese Circulation Soc
- PE-441 Relationship between Matrix Metalloproteinases and Late Gadolinium-Enhancement with Cardiovascular Magnetic Resonance in Patients with Nonischemic Dilated Cardiomyopathy(PE074,Cardiomyopathy/Hypertrophy (Clinical) 2 (M),Poster Session (English),The
- PJ-036 Usefulness of QRS Template Matching in Radiofrequency Catheter Ablation of Idiopathic Right Ventricular Outflow Tract Tachycardia(Arrhythmia, therapy(23)(A),Poster Session(Japanese),The 72nd Annual Scientific Meeting of the Japanese Circulation Soc
- OE-080 A Link between Matrix Metalloproteinases in the Coronary Sinus and Left Ventricular Performance in Patients with Nonischemic Dilated Cardiomyopathy(OE14,Heart Failure (Biomarker) (M),Oral Presentation (English),The 73rd Annual Scientific Meeting of
- PJ-516 Sex Differences in the Relationship between G-protein Beta3 Subunit Gene C825T Polymorphism and Cardiovascular Events in Patients with Dilated Cardiomyopathy(Heart failure, clinical(18)(M),Poster Session(Japanese),The 72nd Annual Scientific Meeting
- Wrapped Alignment Marks for Fabrication of Interference/Diffraction Hot Electron Devices
- W/Cr/Au/SiO_2 Composite Alignment Mark for Fabrication of Interference/Diffraction Hot Electron Devices
- Nanostrueture Alignment for Hot Electron Interference/Diffraction Devices
- Ultrafine Fabrication Technique for Hot Electron Interference/Diffraction Devices
- Analysis of plasma protein in newborn rats treated with diethylstilbestrol by using rapid micro two-dimensional electrophoresis
- EFFECT OF PARAQUAT ON PLASMA FIBRONECTIN, SERUM FREE HYDROXYPROLINE, SERUM CERULOPLASMIN AND LUNG COLLAGEN CONTENT IN MONKEYS
- Decrease in CD4^+CD25^+ and CD8^+CD28^+ T cells in interstitial pneumonitis associated with rheumatic disease
- THE PATTERN OF ACTION OF BLENDED CHINESE TRADITIONAL MEDICINES TO GLUCOSE TOLERANCE CURVES IN GENETICALLY DIABETIC KK-CA^y MICE
- GLUCOSE TOLERANCE CURVES IN GENETICALLY DIABETIC KKCA^y MICE : THE PHARMACOKINETIC ANALYSIS FOR HUMPING EFFECT
- Ontogeny of neuropsin mRNA expression in the mouse brain
- PJ-623 Increased Static Pressure Promotes Collagen Gel Contraction by Cardiac Fibroblasts without Cell Proliferation(Cardiac Hypertrophy, Basic and Clinical 2 (M) : PJ104)(Poster Session (Japanese))
- OE-095 Hypertension Promotes the Phosphorylation of FAK and PYK2 in a Pressure Dependent Manner in Rat Aorta(Molecular Biology, Vascular 1 (H) : OE12)(Oral Presentation (English))
- Kinetics of intraocular cytokines in the suppression of experimental autoimmune uveoretinitis by type I IFN
- P10-04 Changes Detected in Plasma Antiproteases in Paraquat Poisoned Rats Using Micro Two-Dimensional Electrophoretic Analysis
- Experimental and numerical studies on rock bed heat storage for room heating systems
- LAPAROSCOPIC CHOLECYSTECTOMY FOR CHOLECYSTOLITHIASIS IN A CASE WITH SITUS INVERSUS TOTALIS
- Preparation of Diethyl 3-Hydroxy-1-alkenylphosphonates by the Reaction of Diethyl (Arylsulfinylmethyl)phosphonate with Aldehydes under the Knoevenagel Conditions
- NOVEL AND EXTENSIVE ASPECTS OF PARAQUAT-INDUCED PULMONARY FIBROGENESIS : COMPARATIVE AND TIME-COURSE MICROARRAY ANALYSES IN FIBROGENIC AND NON-FIBROGENIC RATS
- Two cases of reactive hemophagocytic syndrome: a patient with adult-onset Still's disease and a patient with herpes zoster and autoimmune abnormalities
- P4-08 Clinicopathological Significance of Cyclin B1 Overexpression in Human Colorectal and Gastric Cancer
- P4-07 MnSOD Expression within Tumor Cells is Closely Related to Mode of Invasion and Histological Differentiation in Human Gastric Cancer
- Laminar Natural Convection Heat Transfer in an Air Filled Square Cavity with Two Insulated Baffles Attached to its Horizontal Walls
- Diffuse laminar endocervical glandular hyperplasia
- CD19/22 balance relates to improvement of disease activity in systemic lupus erythematosus
- A Genetically Diabetic Model “KK-CAy Mice” for a Pharmacological Assay
- Peripheral Pulmonary Artery Aneurysms in Patients with Pulmonary Artery Hypertension
- Proximal Pulmonary Artery Aneurysms in Patients with Pulmonary Artery Hypertension: Complicated Cases
- A307 HEAT TRANSFER CHARACTERISTICS OF FOAM FLOW AT TEMPERATURE UP TO 373K OF HEATING SURFACE(Multiphase flow problems)
- Comparison of Patients with Ocular Tuberculosis in the 1990s and the 2000s
- Proposal of the Atmospheric Pressure Turbine (APT) and High Temperature Fuel Cell Hybrid System(International Conferences on Power and Energy System)
- Uveitis Associated with Sarcoidosis Exacerbated by Etanercept Therapy
- Impact of Synergistic Polymorphisms in Adrenergic Receptor-Related Genes and Cardiovascular Events in Patients With Dilated Cardiomyopathy
- Comparative Results of Coronary Intervention in Patients With Variant Angina Versus Those With Non-Variant Angina.
- DOCTOR'S DELAY IN ENDOBRONCHIAL TUBERCULOSIS
- Obstruction of the Superior Mesenteric Artery Due to Emboli from the Thoracic Aorta in a Patient with Thromboangiitis Obliterans
- Successful treatment with rituximab of an infant with refractory autoimmune hemolytic anemia