尿酸結晶誘発性炎症におけるTREM-1の作用の解析

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概要

• 論文の詳細を見る

• Triggering receptor expressed on myeloid cells(TREM)-1 is a cell surface molecule that has been identified on monocytes and neutrophils and is implicated in the early inflammatory response induced by microbes. However, it has been remained unclear how the expression of TREM-1 is regulated in non-microbial inflammatory diseases. This study was undertaken to evaluate the biologic role of TREM-1 in the initiation of acute attacks of gout. We investigated TREM-1 expression by monosodium urate monohydrate(MSU) crystal-stimulated resident murine peritoneal macrophages(RPM), and assessed whether TREM-1 signaling amplified response to MSU crystals. TREM-1 expression by RPM stimulated with various inflammatory agents was determined by quantitative real-time PCR and western blot analysis. Cytokine production by RPM cultured with both anti-TREM-1 agonist antibody and MSU crystals was assayed by ELISA. TREM-1 expression was significantly induced in RPM by MSU crystals. The peaks of TREM-1 transcript and product occurred rapidly after 1 and 4 hours of MSU crystal stimulation, respectively. The level of TREM-1 transcript induced was consistent with the result obtained using LPS, a potent TREM-1 inducer. Induction of TREM-1 by anti-TREM-1 agonist Ab and MSU crystals synergistically increased production of IL-1 β and MCP-1 by RPM compared with MSU crystals alone. These findings indicate that rapid induction of TREM-1 expression in RPM by MSU crystals may contribute to the onset of acute gouty arthritis, followed by induction of proinflammatory cytokines.

• 日本炎症・再生医学会の論文
• 2005-05-25

著者

• 北里 英郎

  北里大学 医療衛生学部微生物学教室

• 村上 洋介

  北里大学大学院医療系研究科環境感染学

• 北里 英郎

  北里大学大学院医療系研究科環境微生物学

• 赤星 透

  北里大学大学院医療系研究科臨床検査診断学

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