生体染色と感染実験による Enteromyxum leei の海水中における生残能力の推定
スポンサーリンク
概要
- 論文の詳細を見る
A vital staining assay using Hoechst 33342 and propidium iodide was developed for the determination of viability of Enteromyxum leei (Myxozoa), a causative agent of myxosporean emaciation disease. Using this in vitro viability assay combined with in vivo infectivity test, the survival of developmental stages of E. leei in seawater was evaluated. Developmental stages of E. leei were freshly isolated from infected tiger puffer Takifugu rubripes and incubated in filtered natural seawater at 20ºC for 0, 6, 12 and 24 h. The in vitro staining assay showed a time-dependent decrease in viability, and no viable stages were detected at 24 h. In the first trial of in vivo infectivity, naïve tiger puffer were exposed to E. leei suspensions which were incubated in seawater for 0-48 h. In the second trial, naïve grass puffer T. niphobles were fed E. leei incubated for 0-24 h. After rearing for 3-4 weeks, infection with E. leei was detected in fish challenged with the parasite incubated for 24 and 6 h in the first and second trials, respectively. Results of the in vitro and in vivo assays showed that the survival time of developmental stages of E. leei in seawater was variable but estimated less than 24 h.
- 日本魚病学会の論文
- 2009-12-15
著者
-
小川 和夫
Department of Aquatic Bioscience, Graduate School of Agricultural and Life Sciences, The University
-
小川 和夫
Department Of Aquatic Bioscience Graduate School Of Agricultural And Life Sciences The University Of
-
横山 博
Department Of Aquatic Bioscience Graduate School Of Agricultural And Life Sciences The University Of
-
柳田 哲矢
Department of Parasitology, Asahikawa Medical College
-
蔭山 栄裕
Department of Aquatic Bioscience, Graduate School of Agricultural and Life Sciences, The University
-
柳田 哲矢
Department Of Parasitology Asahikawa Medical College
-
蔭山 栄裕
Department Of Aquatic Bioscience Graduate School Of Agricultural And Life Sciences The University Of
-
柳田 哲矢
Department of Aquatic Bioscience, Graduate School of Agricultural and Life Sciences, The University of Tokyo
関連論文
- ハモの鰾内における Aerobiotrema 属吸虫の寄生
- アサリに寄生する Perkinsus olseni と P. honshuensis のPCR-RFLPによる識別法の開発
- Neoheterobothrium hirame (Monogenea) alters the feeding behavior of juvenile olive flounder Paralichthys olivaceus
- 単生類 Heterobothrium okamotoi 孵化幼生の行動特性
- 魚介類の寄生虫病に関する研究
- 単生類 Neoheterobothrium hirame のサザンフラウンダーへの実験感染
- ニホンウナギとヨーロッパウナギの単生類シュードダクチロギルス寄生に対する感受性の比較
- 種苗生産過程のスジアラ受精卵およびふ化仔魚に認められた Ichthyodinium 属原虫感染症
- 低水温下での Neoheterobothrium hirame の着定と発育
- 日本海西部海域の天然0歳ヒラメにおける Neoheterobothrium hirame の寄生動態