細胞培養法

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Cell culture has become a routine laboratory technique. In recent years, many materials for cell culture have become commercially available. Cells are grown and maintained at an appropriate temperature and gas mixture (typically, 37°C, 5% CO2) in a cell incubator. Culture conditions vary widely for each cell type, and variation of the conditions for a particular cell type can result in the expression of different phenotypes. Some cells naturally live without attaching to a surface, such as cells that exist in the bloodstream. Others require a surface, such as most of the cells derived from solid tissues. Cells growing unattached to a surface are referred to as suspension cultures. Adherent cultured cells can be grown on tissue culture plastic, which may be coated with extracellular matrix components (e.g. collagen or fibronectin) to increase the cellular adhesion properties and provide other signals needed for growth. As cells generally continue to divide in culture, they generally grow to fill the available area or volume. Cell-to-cell contact can stimulate cell cycle arrest, causing the cells to stop dividing, known as contact inhibition. Cell-to-cell contact can stimulate promiscuous and unwanted cellular differentiation. These issues can be dealt with using tissue culture methods that rely on sterile techniques. In this paper, several kinds of cell culture methods and inner ear tissue culture are introduced.
日本めまい平衡医学会の論文
2008-02-01

細胞培養法

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