超高速液体クロマトグラフィーによる有機酸のポストカラム分析

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概要

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• An ultra high-speed liquid chromatograph (UHLC) offers a reduction in the analysis time compared with conventional HPLC by using a column with finer particles and an apparatus resistant to high pressure. Although postcolumn methods are excellent techniques for the analysis of samples containing contaminants, such as foodstuffs, the postcolumn method has not been applied to UHLC because of problems associated with diffusion of the target peak and the reactivity. A postcolumn method using UHLC has not been reported due to some problems such as diffusion of the sample in a reaction part. We successfully applied a postcolumn method using UHLC through optimization of the instrumental system and the analytical conditions with Ascentis Express RP-Amide and LaChromUltra C18-AQ columns. This afforded the high-speed analysis of 10 organic acids ; tartaric acid, formic acid, malic acid, lactic acid, acetic acid, pyroglutamic acid, citric acid, succinic acid, propionic acid and fumaric acid ; within 4 min with good separation and reproducibility. Their limits of detection (S/N = 3) were between 9 to 29 mg/L. The results of measurements of food samples by the postcolumn method using UHLC were similar to those obtained by the postcolumn method using HPLC. Therefore, it can be said that effective measurements are possible using the postcolumn method by UHLC, and that this system is particularly useful for materials that contain large amounts of contaminants, such as foodstuffs.

• 2010-03-05

著者

• 佐々木 翠

  株式会社日立ハイテクノロジーズ那珂アプリケーションセンタ

• 石川 昌子

  株式会社日立ハイテクノロジーズ那珂アプリケーションセンタ

• 鈴木 裕志

  株式会社日立ハイテクノロジーズ那珂アプリケーションセンタ

• 中川 裕章

  株式会社日立ハイテクノロジーズ那珂アプリケーションセンタ

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