酸素及び過酸化水素のポリジメチルシロキサン膜透過性を利用するアンペロメトリックグルコースセンサー

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The permeability for oxygen and hydrogen peroxide through a poly(dimethylsiloxane) (PDMS) layer with various thicknesses on a platinum electrode was investigated in order to develop a glucose sensor based on the detection of a reduction current of oxygen, which was consumed by a catalytic reaction of glucose oxidase (GOx) in the presence of glucose. PDMS layers with various thicknesses were prepared on platinum electrodes by casting various concentrations of the PDMS emulsion. Oxygen and hydrogen peroxide permeated through the PDMS layers on a Pt electrode were detected by cyclic voltammetry in buffer solutions. The PDMS layer allowed us to permeate the oxygen, but also acted as a barrier for the permeation of hydrogen peroxide. The concentration of oxygen in the PDMS layers was roughly estimated by quantitative analysis using an amperometric technique, and was found to be >2.5 mM, which is >10-times higher than that in an air-saturated buffer solution with 0.22 mM oxygen. Finally, PDMS-modified Pt electrodes combined with a poly(vinyl alcohol) stilbazole quaternized (PVA-SbQ) membrane containing GOx were fabricated, which were applied to glucose sensing. The incorporation of PDMS layers with oxygen permselectivity to glucose sensing enhanced the current responses for oxygen reduction because of a restriction of the permeation for hydrogen peroxide and the high concentration level in PDMS. However, the response time increased with increasing the thickness of the PDMS layer. Therefore, a PDMS layer with 50 μm − 100 μm is suitable to fabricate glucose sensors based on the detection of oxygen.
2009-07-05

酸素及び過酸化水素のポリジメチルシロキサン膜透過性を利用するアンペロメトリックグルコースセンサー

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