Phylotype-Specific Productivity of Marine Bacterial Populations in Eutrophic Seawater, as Revealed by Bromodeoxyuridine Immunocytochemistry Combined with Fluorescence in situ Hybridization

スポンサーリンク

概要

• 論文の詳細を見る

• Among the fundamental questions in marine microbial ecology are which taxa or phylogenetic groups account for total bacterial productivity and what is the relative contribution of each. We combined bromodeoxyuridine (BrdU) immunocytochemistry and fluorescence in situ hybridization (BIC-FISH) to examine phylotype-specific contributions to total bacterial productivity in eutrophic seawater. We also examined year-round changes in phylotype-specific contributions and explored the factors controlling these changes. Monitoring by BIC-FISH throughout the year revealed the importance of the Roseobacter/Rhodobacter group as a constantly proliferating basic population (27% of all BrdU-positive cells), although their contribution was not significantly correlated with water temperature or with chlorophyll a or organic matter concentration. The Bacteroidetes were another important group, as they greatly increased in abundance after the end of phytoplankton blooms. Two other phylotypes tested, the SAR86 and Vibrio groups, changed their contributions to bacterial productivity with changes in water temperature. To our knowledge, this study was the first to estimate the yearly contribution of major subgroups of marine bacteria to total bacterial productivity in a seawater environment.

• 日本微生物生態学会・日本土壌微生物学会の論文
• 2009-12-01

著者

• HAMASAKI KOJI

  Ocean Research Institute, University of Tokyo

• Hamasaki Koji

  Ocean Research Institute University Of Tokyo

• Taniguchi Akito

  Laboratory Of Environmental Science For Aquaculture Graduate School Of Agriculture Kinki University

• Taniguchi Akito

  Department Of Fisheries Faculty Of Agriculture Kinki University

• Taniguchi Akito

  Department Of Applied Chemistry Faculty Of Engineering Okayama University Of Science

• TADA YUYA

  Ocean Research Institute, University of Tokyo

• Tada Yuya

  Ocean Research Institute University Of Tokyo

• Hamasaki Koji

  Atmosphere And Ocean Research Institute The University Of Tokyo

関連論文

• Seasonal variations in abundance and biomass of picoplankton in an oyster-farming area of northern Japan
• 2P-1001 Phylogenetic studies on five novel marine bacteria
• Analysis of Nanoplankton Community Structure Using Flow Sorting and Molecular Techniques
• Phylotype-Specific Productivity of Marine Bacterial Populations in Eutrophic Seawater, as Revealed by Bromodeoxyuridine Immunocytochemistry Combined with Fluorescence in situ Hybridization
• Preparation of Diethyl 3-Hydroxy-1-alkenylphosphonates by the Reaction of Diethyl (Arylsulfinylmethyl)phosphonate with Aldehydes under the Knoevenagel Conditions
• Analysis of Nanoplankton Community Structure Using Flow Sorting and Molecular Technique
• Seasonal Variations in the Community Structure of Actively Growing Bacteria in Neritic Waters of Hiroshima Bay, Western Japan
• Seasonal Variations in the Community Structure of Actively Growing Bacteria in Neritic Waters of Hiroshima Bay, Western Japan

スポンサーリンク