Estrone Sulfate and Progesterone Profiles During Late Gestation in Recipient Cows Transferred Embryos Produced by Nuclear Transfer and In Vitro Fertilization

概要

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The aim of present experiment was to evaluate the plasma concentrations of estrone sulfate (E1S) and progesterone (P4) during late gestation in recipient cows transferred embryos produced by nuclear transfer (NT) and in vitro fertilization (IVF). Blood samples were collected from recipients transferred embryos produced by NT (n=9) and IVF (n=13) at 160, 220, 240, 260 and 270 d of gestation and then at 5 d intervals until parturition. Plasma samples were analyzed for E1S and P4 by ELISA. One NT and three IVF cows aborted between days 220 and 260 of gestation. Two NT and one IVF cow had prolonged gestation (over 290 d). One IVF cow had an overweight fetus (50 kg) after abortion (257 d). The patterns of changes in the concentrations of E1S during late gestation in the NT and IVF cows were almost identical. The NT and IVF cows that aborted had prolonged gestation and much higher E1S concentrations than the average. One NT cow aborted after 220 d of gestation and had a sudden high increase in its E1S concentration from 160 d to 220 d of gestation. The NT and IVF cows that had prolonged gestation also had significantly higher (p<0.05) P4 concentrations than the average. These results raise the possibility that the E1S and P4 profiles can be used to monitor some late gestational problems, such as higher birth weight, abortion and prolonged gestation.
日本繁殖生物学会の論文
2007-12-01