Multi-component HPLC Fingerprinting of Radix Salviae Miltiorrhizae and Its LC-MS-MS Identification
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概要
- 論文の詳細を見る
The multi-component fingerprinting method of Radix Salviae Miltiorrhizae (Root of Salvia miltiorrhiza BGE.), an important and popular medicinal herb in traditional Chinese medicine, was studied using reversephase HPLC and LC-MS-MS. Extract containing both the water-soluble phenolic compounds and nonpolar diterpenoid compounds known to be the herb's main bioactive components was prepared by a two-step extractive procedure. An HPLC fingerprinting method which can simultaneously separate these two types of compounds was established with gradient elution mode and photodiode array detection at 280nm. Eighteen peaks in this HPLC fingerprint were structurally identified by employing LC-MS-MS techniques. The electrospray ionization (ESI) MS-MS spectra of most salvianolic acids displayed a characteristic behavior of loss of danshensu and caffeic acid moieties, while those of tanshinones showed a particular behavior of loss of H_2O which is quite different from the fragmentation pattern in electron ionization mass spectrometry (EI-MS). The HPLC fingerprints of 7 batches of crude drugs showed similar separation pattern and provided much chemical information of the pharmacologically-active compounds in the crude drugs, which is useful for the authentication and quality evaluation of this medicinal herb.
- 公益社団法人日本薬学会の論文
- 2005-06-01
著者
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Jiang Zhi
School Of Chinese Medicine Hong Kong Baptist University
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Jiang Zhi‐hong
Hong Kong Baptist Univ. Hkg
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Hu Ping
Hong Kong Baptist Univ. Hkg
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Hu Ping
School Of Agriculture And Biology Shanghai Jiaotong University
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Luo Guo‐an
East China Univ. Sci. And Technol. Shanghai Chn
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Luo Guo
School Of Chemistry And Pharmaceutics East China University Of Science And Technology:analysis Cente
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Zhao Zhong
School Of Chinese Medicine Hong Kong Baptist University
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LUO Guo-An
School of Pharmaceutics, East-China University of Science & Technology
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Liang Qiong
Key Laboratory Of Bioorganic Phosphorus Chemistry & Chemical Biology (ministry Of Education) Dep
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LIANG Qiong
Analysis Center, Tsinghua University
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Liang Qiong-lin
Key Laboratory Of Bioorganic Phosphorus Chemistry & Chemical Biology Ministry Of Education Depar
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Jiang Zhi
School Of Chinese Medicine Hong Kong Baptist Univ.
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Liang Qiong-Lin
Analysis Center, Tsinghua University
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Luo Guo-An
School of Chemistry and Pharmaceutics, East China University of Science and Technology:Analysis Center, Tsinghua University
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