Spectrophotometric Determination of Plasma and Red Blood Cell Cholinesterase Activity of 53 Fruit Farm Workers Pre- and Post-Exposed Chlorpyrifos for One Fruit Crop

概要

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We sought to investigate the early biological effects of chlorpyrifos among 53 Thai fruit farm workers by measuring the plasma cholinesterase (PChE) and red blood cell cholinesterase (AChE) activities, a biomarker of organophosphate (OPs) pesticide during one fruit crop. The ChE activity (V_m/K_m) was spectrophotometrically analyzed before and after exposing to chlorpyrifos. The V_m/K_m values of both non-spraying and spraying seasons are found as normal distribution pattern. The median PChE and AChE activities among farm workers in the non-spraying season were 2.3×10^<-3>s^<-1> and 7.26×10^<-5>s^<-1>, respectively. The median PChE and AChE activities of the farm workers in the spraying season were 2.02×10^<-3>s^<-1> and 5.95×10^<-5> s^<-1>, respectively. The mean V_m/K_m values of PChE shifted left (t-test, p=0.013), indicating a decrease in PChE activity in the farm workers exposed to chlorpyrifos. However, the V_m/K_m values of AChE in nonspraying season and in the spraying season were not different (t-test, p=0.246). We propose that PChE activity can be used as a biomarker for monitoring early toxicity induced by chlorpyrifos insecticide.
公益社団法人日本薬学会の論文
2005-04-01