Hydroxyapatite 顆粒の歯周治療への応用に関する神経病理組織学的研究 : 顆粒サイズの違いが神経再分布に及ぼす影響について

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The purpose of the present study was to evaluate periodontal wound healing and the regenerative nerve responses following the implantation of different sized porous Hydroxyapatite granules (HAP) (large size : 600 to 1000μm, small size : 100-400μm) in the experimental periodontal defects of 21 mongrel dogs. Three-wall bone defects (3mm×3mm×3mm) were prepared at the second and the fourth premolars for this experiment following a healing period of 3 months after extracting the bilateral 3rd premolars in the mandible. Either the large or small sized HAP granules were implanted in the left and the right side alternatively. Thereafter, the dogs were sacrificed at 1 day, 3 days, 1 week, 2 weeks, 1 month, 3 months and 6 months postoperatively. The sections were examined with hematoxylin-eosin stain and Ungewitter's silver nitrate method based on the modification of Powers, and they were also observed by a light microscopic analysis. In addition, a histomorphometrical analysis was carried out for the period from 1 month to 6 months. The results were as follows : 1. The healing process was similar in both groups. 2. A long junctional epithelial attachment was observed in both groups. There was no difference on the length of epithelial attachment in both groups. 3. In new bone formation the HAP granules were observed to conjugate with each other in the new bone. In addition, the HAP granules were suggested to have such osteogenic activities as osteoconduction and osteoinduction. 4. Regarding the histomorphometrical analysis, the small size group showed a larger amount of new bone formation than the large size group. 5. Ankylosis formed between the tooth and new alveolar bone. The new ligament was found to be different from the normal structure. 6. The regenerative nerve innervation was influenced by the new bone formation and also showed a difference between the small and large size groups. 7. The regenerative nerve (inferior alveolar nerve) terminals were observed to come in contact with the undifferentiated mesenchymal cells and multinucleated giant cells that adhered to the HAP granules in both groups. As a result, the nerves may possibly be related to the bone formation. As described in the above study, in order to accurately evaluate bioactive materials for application in periodontal therapy, the use of neurohistological studies are indispensable.
九州歯科学会の論文
2000-10-25

Hydroxyapatite 顆粒の歯周治療への応用に関する神経病理組織学的研究 : 顆粒サイズの違いが神経再分布に及ぼす影響について

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