Comparison of Traditional Culture Method with DOX System for Detecting Coliform and Escherichia coli from Vegetables
スポンサーリンク
概要
- 論文の詳細を見る
The methods of sanitary inspection performed primarily by traditional culture methods are time consuming, laborious, and require complicated laboratory techniques. A number of instrumental methods have been introduced for the rapid determination of viable cells based on the metabolic activities of microorganisms. The DOX system is one of the newly developed rapid inspection methods. It has three independent incubators that hold 60 vials each can estimate viable bacterial cell number by measuring the respiration speed of microorganisms and also has a high correlation between the detection time (Dt) and the viable bacterial cell number. We made a quantification analysis of coliform, and identified the presence of Escherichia coli from various kinds of vegetable samples. A total of 131 vegetable samples were tested to compare the plate count of coliform to the count using the DOX method. The correlation coefficient for the estimation of coliform-bacterial cell number was 0.85, which theoretically suggested that the DOX system was able to detect one cell of coliform-bacteria after 17.7 h of incubation. Among the 131 samples, 20 samples were confirmed for the presence of E. coli-positive cells using the UV-lamp method. This study suggested that the DOX system performs better than the conventional method for the routine inspection of coliform and E. coli from vegetable samples. Therefore, this system could be useful for the rapid detection of coliform and E. coli from vegetable samples with minimum labor and cost.
- 社団法人 日本食品科学工学会の論文
- 2003-08-01
著者
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Isshiki K
National Food Research Institute Food Hygiene Research Team
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Isshiki Kenji
National Food Research Institute
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Amano Yoshihisa
Daikin Environ. Lab.
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Kawasaki S
National Food Research Institute Food Hygiene Research Team
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KAWASAKI Susumu
National Food Research Institute, Food Hygiene Research Team
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NAZUKA Eiichi
National Food Research Institute, Food Hygiene Research Team
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BARI Latiful
National Food Research Institute, Food Hygiene Research Team
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YOSHIDA Masakazu
Daikin Environmental laboratory, Limited
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Amano Yoshihisa
Daikin Environmental Laboratory Limited
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Bari Latiful
National Food Research Institute
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Yoshida Masakazu
Daikin Environmental Laboratory Limited
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Bari Latiful
National Food Research Institute Food Hygiene Research Team
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Nazuka Eiichi
National Food Research Institute Food Hygiene Research Team
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Kawasaki Susumu
National Food Research Institute National Agriculture And Food Research Organization
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