Mechanisms Underlying the Induction of Vasorelaxation in Rat Thoracic Aorta by Sanguinarine
スポンサーリンク
概要
- 論文の詳細を見る
In the present study, the effect of sanguinarine (SANG) on smooth muscle was investigated in thoracic aorta isolated from rats. SANG dose-dependently relaxed the phenylephrine (PE, 3 μM)-precontracted aorta; and the concentrations to produce 50% relaxation were 3.18 ± 0.37 and 3.42 ± 1.14 μM, respectively, in intact and denuded aorta. These results suggest that the relaxing effect of SANG was endothelium-independent. The total contraction induced by PE was inhibited in aorta pretreated with SANG at μM concentration. Both phasic and tonic contractions induced by PE were inhibited by SANG independently, which were further supported by the fact that inositol 1,4,5-trisphosphate (IP3) formation and 45Ca2+ influx induced by 3 μM PE in denuded aorta were inhibited by SANG concentration-dependently. In addition, the vasocontraction induced by high-K+ was also inhibited by SANG, however, at higher concentrations. The inhibitory effects of SANG were reversed by dithiothreitol, a thiol reducing agent, implying that the oxidation of critical sulfhydryl groups on key molecules that regulate the smooth muscle contraction were involved. These data suggested that the inhibitory effects of SANG on PE-induced vasocontraction might involve the inhibition of IP3 formation and blockade of calcium channel.
- 社団法人 日本薬理学会の論文
- 2001-01-01
著者
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Cheng Hui
Institute Of Dermatology & Department Of Dermatology The First Hospital Anhui Medical University
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Kang Jaw
Institute Of Toxicology College Of Medicine National Taiwan University
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Cheng Yu
Institute Of Pharmaceutical Sciences Taipei Medical University
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Cheng Hui
Institute Of Pharmaceutical Sciences Taipei Medical University
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Hu Chien
Institute Of Pharmaceutical Sciences Taipei Medical University
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