RAPD, RFLP and SSLP analyses of phylogenetic relationships between cultivated and wild species of rice

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概要

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• RAPD, RFLP, nuclear SSLP and chloroplast SSLP analyses were carried out to clarify the phylogenetic relationships among A-genome species of rice. In total, 12 cultivars of Oryza sativa (4 Japonica, 3 Javanica and 5 Indica), one cultivar of O. glaberrima, and 17 wild accessions (12 O. rufipogon, 2 O. glumaepatula, 1 O. longistaminata, 1 O. meridionalis and 1 O. barthii) were used. Their banding patterns were scored and compared to evaluate the similarity between accessions. Genetic differentiation within and between taxa was examined based on the average similarity indices Except for chloroplast SSLP analysis, the average similarities were higher within O. sativa than within O. rufipogon, and O. sativa Indica had greater intrasubspecific variation than Japonica and Javanica. Comparisons between cultivated and wild species showed that O. sativa was closely related to O. rufipogon, while O. glaberrima was closely related to O. barthii. This indicated that two cultivated species, O. sativa and O. glaberrima, originated from O. rufipogon and O. barthii, respectively. Domestication of O. sativa seemed to be diphyletic, since strong similarity was observed between O. sativa Japonica-Javanica and O. rufipogon from China and between O. sativa Indica and O. rufipogon from tropical Asia. In addition, dendrograms for RAPD, RFLP, and nuclear and chloroplast SSLP analyses were constructed to reval the overall genetic relationships among A-genome species. In all analyses, O. sativa and O.glaberrima formed groups with O. rufipogon and O. barthii, respectively. However, their manners of clustering with other wild species were not the same. The results of RAPD and RFLP analyses indicate that O. glumaepatula was relatively close to the groups of O. sativa and O. glaberrima whereas O. longistaminata and O. meridionalis were highly differentiated from other A-genome species. On the other hand, clear interspecific relationships were not obtained by nuclear or chloroplast SSLP analyses.

• 日本遺伝学会の論文

著者

• Kamijima Osamu

  Laboratory Of Plant Breeding Faculty Of Agriculture Kobe University

• ISHII Takashige

  Laboratory of Plant Genetics, Graduate School of Agricultural Science, Kobe University

• Solis R

  Philippine Rice Research Institute

• BAUTISTA Nonnatus

  Graduate School of Science and Technology Faculty of Agriculture, Kobe University

• SOLIS Renando

  Philippine Rice Research Institute

• Ishii Takashige

  Laboratory Of Plant Breeding Faculty Of Agriculture Kobe University

• Bautista Nonnatus

  Graduate School Of Science And Technology Faculty Of Agriculture Kobe University

• ISHII Takashige

  Laboratory of Genetics, Faculty of Agriculture, Kyoto University

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